It is unlikely that changes in channel expression underlie the appearance of TRPV4 channels in muscarinic receptor signaling given that (1) no differences were observed in degree of co-localization between TRPV4 and either Cav-1 or eBK and (2) direct activation of TRPV4 elicited a similar vasodilation between arteries from control and CH rats. The gene discussed is TRPV4; the disease is cyclic hematopoiesis.