Growing the mutant cells in nominally iron-free medium for 24 h strongly inhibited Legionella intracellular growth, whereas cell growth under iron overload enhanced its susceptibility to infection to a higher level than in the parental cells, consistent with a higher accumulation of iron in the LCV, due to inactivation of Nramp1 (Buracco et al., 2015). Here, SLC11A1 is linked to infection.