In summary, we reported that the hypouricemia effects of G. applanatum extract in hyperuricemia mice were attributed to the regulation of renal OAT1, GLUT9, and URAT1 and gastrointestinal CNT2 for GAE and OAT1 and GLUT9 and gastrointestinal CNT2 for GAW. This evidence concerns the gene SLC22A12 and hyperuricemia.