We use BRAF V600E mutant melanoma cells because (1) bleb-based forms of migration have been observed both in vivo and in vitro [12, 15, 20], (2) melanoma often metastasizes non-hematologically, more specifically, not carried to distant tissues by the circulatory system [21], and (3) melanoma has an exceptionally high morbidity rate after it has metastasized. This evidence concerns the gene BRAF and melanoma.