To determine if this epigenetic mechanism contributes to downregulation of DUSP5 in CRCs, we used bisulphite conversion and sequencing to interrogate 4 contiguous regions spanning the DUSP5 proximal promoter, exon 1, and the first part of intron 1, in a panel of 25 CRC cell lines (Fig. 3A). This evidence concerns the gene DUSP5 and colorectal carcinoma.