Given that the TNF‐α/NF‐κB axis was activated in osteosarcoma cells, we next sought to investigate whether the overexpression of NF‐κB subunits in hFOB1.19 cells would cause CUL4B up‐regulation and p21 down‐regulation similar to that of osteosarcoma cells. Here, CDKN1A is linked to osteosarcoma.