To further evaluate the presence and distribution of the identified two susceptibility loci including NRG1 and AUTS2, we collected tissue samples from 54 HSCR patients to pairwise compare the expression level of targeted gene in the aganglionic segments (narrow) and normal segments (dilated) using qRT‐PCR as shown in Figure 1. This evidence concerns the gene AUTS2 and Hirschsprung disease.