Because global gene expression and pathway analyses indicated that members of the Rho GTPase family participated in some pathways to regulate the cytoskeleton and invasion ability of breast cancer cells, we used western blots to verify RhoA, Rac1, Cdc42 and ROCK1protein expression in AmotP130 up‐ or down‐regulated cells (Figure 5A). This evidence concerns the gene CDC42 and breast cancer.