The model has demonstrated in vivo the genetic requirement for Twist expression downstream of BRAFV600E, as ablation of twist3 by CRISPR-Cas9 suppressed BRAF-mediated oncogenesis.49 The in vivo validation of targeted molecular therapies for the treatment of thyroid cancer demonstrates the applicability of the zebrafish system to precision oncology approaches. Here, BRAF is linked to thyroid gland carcinoma.