We randomly selected CG sites that were significantly different between HCC and controls in T cells that were either hypermethylated A Kinase (PRKA) Anchor protein 7 (AKAP7) gene, the Signal Transducing Adaptor Family 1 (STAP1), or hypomethylated the Schlafen family member 14(SLFN14) gene for validation using pyrosequencing (Fig. 6a). This evidence concerns the gene STAP1 and hepatocellular carcinoma.