In our study, DAT methylation at CpG sites M1, M6, and M7 in mothers was correlated with maternal Anxiety and Phobic Anxiety, whereas methylation in fathers at CpG M6 was related to paternal Depression, Anxiety, Hostility, Psychoticism and a higher score in GSI at SCL-90-R. This evidence concerns the gene SLC6A3 and major depressive disorder.