When the expression of EMT molecules were studied with the HCC cell lines by qRT-PCR and Western blotting, we observed that the expression of EMT related genes (MMP-2, MMP-9, E-caherin and vimentin) significantly changed as a result of interfering or overexpression of ALCAM (Figures 6D–H). The gene discussed is ALCAM; the disease is hepatocellular carcinoma.