Whereas centrally extracted DNA enabled highly consistent detection of all variants irrespective of the NGS method applied, the use of locally processed and extracted DNA from the Colon cancer samples prevented the detection of some variants in two samples: ColonCa#1, KRAS mutation (G13D) at sequencing site d, and ColonCa#2, KRAS mutation (G12V) at sequencing site e. This evidence concerns the gene KRAS and colonic neoplasm.