In addition to the confirmation of pre-characterized mutations (Figs. 3 and 4), additional variants were consistently detectable by commercial amplicon panel-based NGS, including variants in TP53, APC, ATM, PIK3CA, and JAK3. Frequencies for all additionally detected variants at all sequencing sites using the commercial cancer panels are listed in supplementary Table 4. The gene discussed is PIK3CA; the disease is cancer.