We found that the combination index of combined treatment with TAK-165 and AC220 on ES-2 cells (Fig. 3a) and on two AML cell lines, HEL (Fig. 3b) and OCI-AML3 (Fig. 3c) all showed a strong combination index of below 0.3 (Fig. 3d), suggesting that TAK-165 has a very strong synergistic effect with AC220 in promoting the cytotoxicity. The gene discussed is RUNX2; the disease is acute myeloid leukemia.