shCTRL cells and Anxa3 knockdown 4T1 cells were implanted to the fourth mammary glands of BALB/c mice, and each group was divided into two groups for different treatments when the tumor size reached approximately 2–3 mm in diameter: one group was treated with saline (control) and the other group was treated with Dox. This evidence concerns the gene ANXA3 and neoplasm.