RHOA and breast carcinoma: For this, IMCD cells, mouse collecting duct MCD4 cells (another model for renal principal cells [44, 48]), Human embryonic kidney (HEK)293, breast carcinoma (MCF7) and cardiac myocytes (H9C2) that all express AKAP-Lbc and RhoA (Fig 6A) were incubated with Scaff10-8 in concentrations of 3–100 μM and an MTS cytotoxicity assay was carried out (Fig 6B).