In the rat osteosarcoma cell line UMR-106, which is capable of osteogenic differentiation, PACAP38 (agonist) and PACAP6-38 (antagonist) were able to enhance osteogenic differentiation by induction of collagen type I, osterix and ALP expression, increased nuclear expression of Runx2 and elevated deposition of inorganic matrix components into the extracellular matrix [125]. The gene discussed is ADCYAP1; the disease is osteosarcoma.