ROC analysis of APPAT revealed an AUC of 0.9302 (95% confidence interval CI [0.8795–0.9810], P < 0.0001) (Fig. 4A) in MI group, whereas AUC of cTnI was 0.8951 (95% CI [0.8247–0.9655], P < 0.0001). This evidence concerns the gene TNNI3 and myocardial infarction.