For the 121 candidate CTCF-binding sites, MS-HRM analysis identified 23 CTCF-binding sites with methylation profiles highly specific in tumor tissues and were, thus, considered as potential candidates for colorectal neoplasia biomarkers (Supplementary Table 1); the remaining 98 CTCF-binding sites were eliminated because they did not exhibit impressive tumor-specific methylation profiles. This evidence concerns the gene CTCF and neoplasm.