We identified that TNM stage (P=0.016), tumor number (P=0.012), AFP>400 (ng/ml) (P=0.008), MVI (P=0.024), tumor size (P<0.001) and under-expression of MIR22HG (P=0.021) were significant prognostic factors associated with HCC patients (Table 2). The gene discussed is AFP; the disease is hepatocellular carcinoma.