Therefore, in order to explore the potential mechanism by which LKB1 loss cooperates with BRAF V600E to promote melanoma cells invasion and migration, MMP-2 and PI3K/Akt/mTOR mediated signal molecules were investigated by using Western blot analysis in A375 cells, which transfected with siLKB1 and siCtrl respectively. This evidence concerns the gene AKT1 and melanoma.