BEND5 overexpression in DLD-1 colon cancer cells and BEND5 knockdown in COLO 320 DM colon cancer cells indicated that BEND5 could significantly change their expression levels (Figure 5E), suggesting that BEND5 may directly or indirectly regulate CCNB1, PCNA and BCL2. Here, BCL2 is linked to malignant colon neoplasm.