In contrast, 16 proteins including annexins (ANXA1 and ANXA5), serpins (SERPA1, SERPB3 and SERP5), tropomyosins (TPM3 and TPM4), cofilins (CFL1 and CFL2), hemoglobulin, Peptidyl-prolyl cis-trans isomerase, Proteasome activator complex subunit, thioredoxin, 14-3-3 zeta, S100A7, heat shock protein A5 were significantly upregulated in tongue tumor samples compared with normal tissue samples. The gene discussed is CFL1; the disease is tongue neoplasm.