In addition, conjugating TNF-α to a Cys-Asn-Gly-Arg-Cys (NGR) peptide (recognizing an aminopeptidase N (CD13) isoform on tumor-associated ECs) led to increased adhesion molecule expression on ECs, increased CD8+ T cell infiltration in B16-OVA melanoma, and improved outcome of both ACT (with OVA-specific in vitro-activated OT-1 cells) and DC-OVA vaccine approaches106. This evidence concerns the gene CD8A and neoplasm.