Furthermore, it has been reported that 27HC promotes proliferation of ERα-positive LNCaP prostate cancer cells via ERβ (Lau et al. 2000, Raza et al. 2017) which may account for the apparent discrepancy between ERE reporter assay and cell proliferation responses in RL95 cells observed in the current study. This evidence concerns the gene ESR1 and Familial prostate cancer.