The diagnostic power of NfL (AUC 0.926 ± 0.016) and t-tau (AUC 0.939 ± 0.014) was comparable in terms of sensitivity and specificity in the overall discrimination of prion disease from other NDs, whereas the t-tau/p-tau ratio showed the highest accuracy in this differential diagnosis (AUC 0.982 ± 0.09) (Fig. 1). Here, NEFL is linked to prion disease.