NEFL and Alzheimer disease: However, in the specific comparison between prion disease and AD, NfL showed higher diagnostic value (AUC 0.981 ± 0.007) than t-tau (AUC 0.901 ± 0.021), and the NfL/p-tau ratio yielded the highest accuracy (AUC 0.994 ± 0.004), with 96.2% sensitivity and 95.9% specificity (see Additional file 1: Figure S1).