To gain a more detailed understanding of the phenotypic differences of the MCMV-specific CD8+ T cells that develop during the low, intermediate, and high-dose infection, we used a novel analysis platform, termed Cytosplore (34), for immune cell phenotyping that incorporates approximated t-distributed stochastic neighborhood embedding (A-tSNE) for dimensionality reduction and—the mean—shift clustering algorithm for subset definition, based on the dimensionality reduced data (37). Here, CD8A is linked to infection.