Therefore, we have quantitatively analyzed and identified the pro-angiogenesis sEphrin-A1/EphA2 system in BCa tissue and subsequently investigated the effect of LEF on BCa cells, microvessel sprouting in aortic ring ex vivo assays, the N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-induced BCa mouse model and a tumor xenograft model in vivo to explore the anti-angiogenesis molecular mechanisms of LEF. This evidence concerns the gene EPHA2 and neoplasm.