To further clarify whether NOP53 is responsible for eIF2α dephosphorylation, two constructs encoding the wt YFP-tagged eIF2α and the phosphorylation-incompetent variant eIF2α-S51A were transiently transfected or co-transfected with Flag-tagged NOP53-N4, followed by mock-infection or HSV-1/F infection. The gene discussed is EIF2A; the disease is infection.