In addition, we employed the detection of TRIB3 ubiquitination status and Western blot analysis revealed that the endogenous TRIB3-associated ubiquitination was increased in NSCLC cells overexpressed GAS5, but the level of TRIB3 protein was decreased, indicating that GAS5 potentiated TRIB3 protein ubiquitination and subsequent degradation (Supplementary Figure 1). Here, TRIB3 is linked to non-small cell lung carcinoma.