PHIP and neoplasm: After establishing the induction of CYP2E1 mRNA by PhIP, we investigated whether CYP2E1 induction resulted in the generation of reactive oxygen species (ROS), since CYP2E1 enzyme is easily uncoupled leading to potent induction of ROS (Jimenez-Lopez and Cederbaum 2005), and ROS production may play an important role in tumor initiation and progression (Cerutti 1985; Slaga et al. 1981; Trush and Kensler 1991).