Therefore, we aimed to contribute to the existing data around liquid biopsy in non-metastatic rectal cancer by assessing feasibility, hurdles and potential clinical implications of using KRAS/BRAF mutations as markers for ctDNA detection in a relatively large prospective series of locally advanced rectal cancer (LARC) patients who were included in a randomised phase II trial (EXPERT-C)25. This evidence concerns the gene BRAF and rectal cancer.