FUS and amyotrophic lateral sclerosis: To develop a human MN model of FUS-ALS, we generated human induced pluripotent stem cells (hiPSCs), by classical retroviral “Yamanaka-factor” reprogramming, from three different FUS-ALS patients carrying diverse NLS mutations (R521C, R521L, R495QfsX527; Fig. 1, Table 1).