Finally, scratch assay showed that knockdown of MAD2L2 accelerated the migration rate of CRC cells (Fig. 3A,C,E, and G), while overexpression of MAD2L2 reduced the cell migration rate and this reduction was reversed by NCOA3 overexpression (Fig. 3B,D,F and H). This evidence concerns the gene MAD2L2 and colorectal carcinoma.