The upregulated protein expressions of NADPH oxidase subunits p22phox (Figures 1(d) and 1(e)), p47phox (Figures 1(d) and 1(f)), and NOX-2 (Figures 1(d) and 1(g)) were observed in HUVECs response to HG/HF, and these changes were prevented by salusin-β knockdown. This evidence concerns the gene NCF1 and hydrops fetalis.