To determine the activation state of monocytes infected with the different strains, we evaluated the mean intensity fluorescence (MFI) expression of a series of activation-related molecules such as TLR2, TLR9, HLA-DR, and the costimulatory molecule ligands, CD80 and CD86, by the different cell populations after infection with L. braziliensis or the L. infantum strains. Here, CD80 is linked to infection.