To confirm whether EMT is essential in DPYD-mediated aggressiveness of HCC cells, we further investigated expression changes of EMT markers in HCCLM3 and SMMC7721 cells with different DPYD expression (HCCLM3-vector, HCCLM3-shDPYD, SMMC7721-vector, and SMMC7721-DPYD-OE). This evidence concerns the gene DPYD and hepatocellular carcinoma.