Besides, multivariate analysis using Cox model, including tumor size, TNM stage, lymph node metastasis, distant metastasis, and PLZF expression showed that only PLZF was an independent prognostic factor for OS in GBC patients (HR, 0.48; 95% CI, 0.28–0.83, P = 0.008, Table 2). The gene discussed is ZBTB16; the disease is metastatic malignant neoplasm in the lymph nodes.