In addition, western blotting results showed that the level of TACC3 protein was robustly upregulated in urinary bladder cancer compared with paired adjacent non-tumor tissues and in most of the bladder cancer cell lines tested compared with the immortalized uroepithelial cells SV-HUC-1 (except HT1197 and TCCSUP cells) (Fig. 1b, c). This evidence concerns the gene TACC3 and neoplasm.