MCM6 and hepatocellular carcinoma: More than fourfold decrease in MCM6 expression was observed in SMMC7721 and HCC-LM3 cells treated with shRNA compared with the control group by qRT-PCR (Student’s t-test, with P < 0.05 for both) and western blotting; shRNA3 was the most effective shRNA and was chosen for further study (Fig. 2b-c).