To show that the induction of death by pro-inflammatory macrophages depends on the activity of miR-146a in the tumor cells, we next cocultured RAW 264.7 macrophages with RENCA (Figure 4C) or CT26 (Figure 4E) cells transfected with anti-miR-146a, and assessed tumor cell death relative to cells transfected with the negative control. Here, DDX53 is linked to neoplasm.