AKT1 and infection: Here, we demonstrate that the direct interaction of phosphorylated PI3K, Akt, and ERK molecules with the subunit E of the V-ATPase V1 domain in response to early infection of L-P RVA strains stimulates endosomal acidification, which is required for uncoating of TLP and delivering of DLP into the cytoplasm to continue the virus life cycle.