EPCAM and non-small cell lung carcinoma: In this study, we synthesized two immunomagnetic nanospheres that were modified with anti-FRα and anti-EpCAM antibodies, respectively, and applied them to CTC assays of NSCLC cell lines; the assay for the FRα+ cells was demonstrated to be both sufficiently sensitive and specific to detect FRα highly expressing NSCLC cell lines of SPC-A-1 and H157 cells.