The CTC-enrichment efficiency of anti-EpCAM-MNs alone or a combination of anti-EpCAM-MNs and anti-FRα-MNs was compared using five types of NSCLC cells lines (1.0 × 105 cells/mL in PBS) that expressed different levels of EpCAM and FRα. The gene discussed is FOLR1; the disease is non-small cell lung carcinoma.