In Ca2+ overload cellular model and mouse AP model induced by caerulein, we found that caerulein triggered SOCE via inducing interaction between STIM1 and Orai1, which activated CaN; CaN then induced the dephosphorylation of TFEB /NFAT and nuclear translocation, which in turn promoted the transcriptional activation of multiple chemokines genes and autophagy associated genes. Here, STIM1 is linked to alkaline phosphatase measurement.