BRG1 nuclear staining was observed in 57 of 77 (74.0%) tumour liver tissue from patients whose level of AFP was greater than 20 ng/ml, 19 of 36 (52.8%) tumour liver tissue from patients whose level of AFP was less than or equal to 20 ng/ml in cohort 2 (P < 0.05). The gene discussed is SMARCA4; the disease is neoplasm.