Because a double-negative feedback is equal to positive feedback and is known for its ability to amplify a response into a self-sustained mode that is independent of the original stimuli, the feedback loop composed of Jun, miR-22 and HuR may minimize miR-22 expression and amplify HuR expression in CRC cells, thus allowing CRC cells to become more autonomous, for example, to reproduce more rapidly and to metastasize to new microenvironments. The gene discussed is JUN; the disease is colorectal carcinoma.