To reveal whether the metabolic role of LIPG is relevant to LIPG-mediated enhancements of basal/EMT, migratory and cancer stem cell features, we created a MCF7 cell line (MCF7- LIPGS149A) stably expressing a catalytically inactive mutant of LIPG (S149A), which has been shown to lose the lipase catalytic activity (Broedl et al., 2003). Here, LIPG is linked to cancer.