FRK and breast cancer: To directly determine the effect FRK on EMT in breast cancer cells, we examined the expression of epithelial and mesenchymal markers in the MDA-MB 231 cells stably expressing either FRK-WT or FRK-Y497F, as well as in FRK knockdown SKBR3 and MCF 7 cells via Real-time PCR and Western blotting analyses.