This difference was reproducible in tumors in the Chicago Genomics Cohort (CHGC) (Supplementary Figure 2) and was also observed in the lung squamous (Figure 2C, 2D; Supplementary Figure 3) and bladder cancers in the TCGA database (Supplementary Figure 4), suggesting that these 15 PMT/PDMT genes might play some roles in the CD8+ T-cell exclusion process in HPV-negative SCCHN. Here, CD8A is linked to urinary bladder carcinoma.