Consistent with previous report, the high-level peak located in 3q26-3q telomere that harboring several well-known cancer genes, such as SOX2 and PIK3CA. In our data, nearly all of SOX2 amplifications were TCNA, which could be further validated in a Japanese ESCC cohort [12]. This evidence concerns the gene SOX2 and esophageal squamous cell carcinoma.